Design of a single protein that spans the entire 2-V range of physiological redox potentials

• DOI: 10.1073/pnas.1515897112
• Publication Date: 2015-12-03T02:03:50Z
• Authors: Parisa Hosseinzadeh; Nicholas M. Marshall; Kelly N. Chacón; Yang Yu; Mark J. Nilges; Siu Yee New; Stoyan A. Tashkov; Ninian J. Blackburn; Yi Lu
• Keywords: Azurin; Metalloprotein

Significance Nature spent millions of years to evolve electron transfer proteins that span a wide range reduction potentials (E°′) under physiological conditions, from −1 V 1 vs. standard hydrogen electrode. Understanding the rules govern such tuning within similar classes metalloproteins enables scientists rationally tune E°′of their catalysts without changing active site. An ultimate test understanding is achieve entire E°′ single protein, feat has not been achieved yet. Herein, we conclusively found can cover 2-V using protein with five mutations and two metal ions. We have also provided explanations for structural features responsible high potential.