Molecular portraits of B cell lineage commitment

• DOI: 10.1073/pnas.152327399
• Publication Date: 2002-07-28T22:26:28Z
• Authors: Markus Müschen; Sanggyu Lee; Guolin Zhou; Niklas Feldhahn; Varun Singh Barath; Jianjun Chen; Cordula Moers; Martin Krönke; Janet D. Rowley; San Ming Wang
• Keywords: PAX5; breakpoint cluster region; Lineage (genetic); Lymphopoiesis

In an attempt to characterize early B cell development including the commitment of progenitor cells lineage, we generated and compared genomewide gene expression profiles human hematopoietic stem (HSCs) pre-B (PBCs) by using serial analysis expression. From more than 100,000 tags collected from CD34 + HSCs CD10 CD19 PBCs, 42,399 unique transcripts were identified in but only 16,786 suggesting that 60% expressed silenced during or after lineage. On other hand, mRNAs receptor (pre-BCR)-associated genes are virtually missing account for 10% transcriptome which also show increased apoptosis-related genes. Both concentration transcriptional repertoire on pre-BCR-related together with marked up-regulation apoptosis mediators PBC might reflect selection a functional pre-BCR within bone marrow. Besides known regulator such as PAX5 , E2A EBF most abundantly PBCs include ATM PDGFRA SIAH1 PIM2 C/EBPB WNT16 TCL1 role has not been established yet development.