Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array

• DOI: 10.1073/pnas.152330199
• Publication Date: 2002-07-28T22:26:28Z
• Authors: Rachel McKendry; Jiayun Zhang; Youri Arntz; Torsten Strunz; Martin Hegner; Hans Peter Lang; Marko K. Baller; Ulrich Certa; Ernst Meyer; Hans-Joachim Günt...; Christoph Gerber
• Keywords: Biomolecule; Molecular biophysics

We report a microarray of cantilevers to detect multiple unlabeled biomolecules simultaneously at nanomolar concentrations within minutes. Ligand-receptor binding interactions such as DNA hybridization or protein recognition occurring on microfabricated silicon generate nanomechanical bending, which is detected optically in situ . Differential measurements including reference an array eight sensors can sequence-specifically targets 80-fold excess nonmatching background and discriminate 3′ 5′ overhangs. Our experiments suggest that the motion originates from predominantly steric hindrance effects depends concentration molecules solution. show cantilever arrays be used investigate thermodynamics biomolecular mechanically, we have found specificity reaction consistent with solution data. Hence permit assays parallel femtomoles 75 nM.