The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from Siraitia grosvenorii

• DOI: 10.1073/pnas.1604828113
• Publication Date: 2016-11-08T03:55:04Z
• Authors: Maxim Itkin; Rachel Davidovich...; Shahar Cohen; Vitaly Portnoy; Adi Doron-Faigenboim; Elad Oren; Shiri Freilich; Galil Tzuri; Nadine Baranes; Shmuel Shen; Marina Petreikov; Rotem Sertchook; Shifra Ben-Dor; Hugo Gottlieb; Alvaro Hernandez; David R. Nelson; Harry S. Paris; Yaakov Tadmor; Yosef Burger; Efraim Lewinsohn; Nurit Katzir; Arthur Schaffer
• Keywords: Epoxide Hydrolases; Models, Molecular; 2. Zero hunger; 0303 health sciences; Gene Expression Profiling; Sequence Analysis, DNA; Triterpenes; Biosynthetic Pathways; Molecular Docking Simulation; Cucurbitaceae; 03 medical and health sciences; Cytochrome P-450 Enzyme System; Squalene Monooxygenase; Gene Expression Regulation, Plant; Glucosyltransferases; Plant Proteins

Significance We identified the biosynthetic pathway for nonsugar sweetener mogroside V, a noncaloric with sweetening strength 250-fold that of sucrose. This compound is produced by fruit endemic Chinese cucurbit Siraitia grosvenoriii , also known as monk and luo-han-guo . The metabolic was using combination genomic transcriptomic databases plant, together large-scale functional expression candidate genes. novelty could be attributed to highly coordinated gene pattern responsible unique epoxidations, hydroxylations, glucosylations leading sweet mogrosides. These discoveries will facilitate development alternative natural sweeteners.