Aquaporin-3 mediates hydrogen peroxide-dependent responses to environmental stress in colonic epithelia

• DOI: 10.1073/pnas.1612921114
• Publication Date: 2017-01-04T01:50:20Z
• Authors: Jay R. Thiagarajah; Jeffrey Chang; Jeremy A. Goettel; Alan S. Verkman; Wayne I. Lencer
• Keywords: 0301 basic medicine; aquaporin-3; Colon; Knockout; Physiological; hydrogen peroxide; Stress; Oral and gastrointestinal; Mice; 03 medical and health sciences; Stress, Physiological; 2.1 Biological and endogenous factors; Innate; Animals; Humans; Aetiology; Intestinal Mucosa; Immunity, Mucosal; Cancer; Mice, Knockout; Mucosal; Aquaporin 3; Wound Healing; 0303 health sciences; colon; Inflammatory and immune system; Inflammatory Bowel Disease; Immunity; Hydrogen Peroxide; Immunity, Innate; Colo-Rectal Cancer; 3. Good health; microbe; Gene Knockdown Techniques; Citrobacter rodentium; Caco-2 Cells; Digestive Diseases; epithelium; Signal Transduction

Significance

The extracellular production of hydrogen peroxide (H 2 O 2 ) by membrane or secreted oxidases has been linked to epithelial wound repair, defense against infection, and inflammation. Here, we elucidate the pathway that explains how extracellular H 2 O 2 transduces a signal into the cell to induce these critical cell functions. Our study shows the central component of this pathway in the mammalian colon is the H 2 O 2 -conducting aquaglyceroporin AQP3 and implicates this channel in innate immune responses at mucosal surfaces.