Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval–adult metamorphosis

• DOI: 10.1073/pnas.1615423114
• Publication Date: 2017-01-18T03:01:19Z
• Authors: Takumi Kayukawa; Akiya Jouraku; Yuka Ito; Tetsuro Shinoda
• Keywords: Male; 0301 basic medicine; Chromatin Immunoprecipitation; Ecdysone; Binding Sites; Kruppel-Like Transcription Factors; Metamorphosis, Biological; Pupa; Gene Expression Regulation, Developmental; Bombyx; Methoprene; Cell Line; 03 medical and health sciences; Protein Domains; Genes, Reporter; Larva; Consensus Sequence; Animals; Insect Proteins; Protein Isoforms; Female; Promoter Regions, Genetic

SignificanceJuvenile hormone (JH) intricately controls molting and metamorphosis in holometabolous insects.Ecdysone-induced protein 93F(E93) functions as an adult specifier gene in the pupal–adult transition. JH is known to repressE93expression to prevent immature larvae from bypassing the pupal stage and progressing to precocious adult development; however, the molecular mechanism underlying JH-mediatedE93repression remains unknown. Here, we demonstrated that JH-inducible Krüppel homolog 1 functions as a direct transcriptional repressor ofE93. This study markedly advances the present understanding of the molecular basis of JH function in repressing insect metamorphosis.