Serum-borne bioactivity caused by pulmonary multiwalled carbon nanotubes induces neuroinflammation via blood–brain barrier impairment

• DOI: 10.1073/pnas.1616070114
• Publication Date: 2017-02-22T01:40:29Z
• Authors: Mario J. Aragon; Lauren Topper; Christina R. Tyler; Bethany Sanchez; Katherine Zychowski; Tamara Young; Guy Herbert; Pamela Hall; Aaron Erdely; Tracy Eye; Lindsey Bishop; Samantha A. Saunders; Pretal P. Muldoon; Andrew K. Ottens; Matthew J. Campen
• Keywords: CD36 Antigens; 0301 basic medicine; Drug Carriers; rho-Associated Kinases; Nanotubes, Carbon; Brain; Endothelial Cells; 3. Good health; Thrombospondin 1; Mice; 03 medical and health sciences; Blood-Brain Barrier; Cell Movement; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Astrocytes; Administration, Inhalation; Animals; Encephalitis; Fluorescein; Lung; Protein Kinase Inhibitors; Fluorescent Dyes

Significance Inhaled particulates, such as multiwalled carbon nanotubes, can induce neuroinflammatory outcomes. The present study shows that acute neuroinflammation is dependent on the impairment of blood-brain barrier function. Pharmacologic restoration integrity prevented responses to pulmonary nanotube exposure. Circulating factors, including possibly thrombospondin-1, recapitulate inflammatory in cultured cerebrovascular endothelial cells, suggesting a mechanism for indirect systemic effects inhaled nanoparticles.