Suboptimal T-cell receptor signaling compromises protein translation, ribosome biogenesis, and proliferation of mouse CD8 T cells

• DOI: 10.1073/pnas.1700939114
• Publication Date: 2017-07-11T00:50:23Z
• Authors: Thomas C. J. Tan; John Knight; Thomas Sbarrato; Kate Dudek; Anne E. Willis; Rose Zamoyska
• Keywords: ribosome biogenesis; Polysome; Ribosome profiling

Significance Optimal antigenic stimulation through T-cell receptors is required by T lymphocytes to exert full expansion, effector functions, and memory cell differentiation. Suboptimal TCR influences both transcription of genes synthesis subsets proteins in a nonconcordant manner. Detailed polysome profiling revealed that weakly activated cells prioritized mRNA translation so specific transcripts were translationally sequestered. Strikingly, ribosome biogenesis was compromised at transcriptional translational levels after weak stimulation, which still allowed the undergo initial division, but proliferation not sustained. Our work has demonstrated respond environmental signals use components machinery regulate activation-dependent mRNAs.