Cap-binding protein 4EHP effects translation silencing by microRNAs

• DOI: 10.1073/pnas.1701488114
• Publication Date: 2017-05-10T00:40:35Z
• Authors: Clément Chapat; Seyed Mehdi Jafar...; Edna Matta-Camacho; Geoffrey G. Hesketh; Idit A. Gelbart; Jan Attig; Christos G. Gkogkas; Tommy Alain; Noam Stern-Ginossar; Marc R. Fabian; Anne-Claude Gingras; Thomas F. Duchaine; Nahum Sonenberg
• Keywords: Nucleocytoplasmic Transport Proteins; 0303 health sciences; Biochemistry; MicroRNAs; 03 medical and health sciences; Eukaryotic Initiation Factor-4E; HEK293 Cells; RNA Cap-Binding Proteins; Journal Article; Humans; RNA-Induced Silencing Complex; RNA Interference; HeLa Cells

Significance miRNAs are important components of gene regulatory networks and affect all aspects cell biology by controlling the stability translation efficiency their target mRNAs. Here, we identified mRNA cap-binding eIF4E-related protein 4EHP as an effector miRNA-mediated repression. Through screening for interactions in cells via BioID method, a component CCR4–NOT/DDX6/4E-T axis. Direct interaction between 4E-T increases latter’s affinity, suggesting that this potentiates its competition with eIF4F complex binding to 5′ cap. Our findings suggest facilitates formation closed-loop structure 3′ UTR cap, which causes repression translation.