Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

• DOI: 10.1073/pnas.1704020114
• Publication Date: 2017-08-01T00:50:22Z
• Authors: Russell H. Cole; Shi-Yang Tang; Christian A. Silt...; Payam Shahi; Jesse Q. Zhang; Sean Poust; Zev J. Gartner; Adam R. Abate
• Keywords: On demand; 3d printed

Significance Single-cell biology requires the ability to construct experiments with single-cell precision, large numbers, and extremely small fluid volumes. To address this need, we have developed a new technology, printed droplet microfluidics, which encapsulates reagents single cells in picoliter droplets, then actively selects deposits desired droplets an arrayed format on printing substrate. This technology enables construction of number relevant single-cellular multicellular methods detection.