Sialylation on O-glycans protects platelets from clearance by liver Kupffer cells

• DOI: 10.1073/pnas.1707662114
• Publication Date: 2017-07-18T01:00:34Z
• Authors: Yun Li; Jianxin Fu; Yun Ling; Tadayuki Yago; J. Michael McDaniel; Jianhua Song; Xia Bai; Yuji Kondo; Yannan Qin; Christopher Hoover; Samuel McGee; Bojing Shao; Zhenghui Liu; Roberto Sonon; Parastoo Azadi; Jamey D. Marth; Rodger P. McEver; Changgeng Ruan; Lijun Xia
• Keywords: Kupffer cell

Most platelet membrane proteins are modified by mucin-type core 1-derived glycans (O-glycans). However, the biological importance of O-glycans in clearance is unclear. Here, we generated mice with a hematopoietic cell-specific loss (HC C1galt1-/- ). These lack on platelets and exhibit reduced peripheral numbers. Platelets from HC show levels α-2,3-linked sialic acids increased accumulation liver relative to wild-type platelets. The preferential was lacking hepatic asialoglycoprotein receptor [Ashwell-Morell (AMR)]. found that Kupffer cells primary phagocytosing liver. Our results demonstrate AMR promotes adherence phagocytosis desialylated and/or cell through its C-type lectin CLEC4F. findings provide insights into an essential role for 1 O-glycosylation their