Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics
0301 basic medicine
0303 health sciences
Placenta
Microfluidic Analytical Techniques
Trophoblasts
Plasma
03 medical and health sciences
Pre-Eclampsia
Pregnancy
Humans
RNA
Female
Single-Cell Analysis
Transcriptome
Cell-Free Nucleic Acids
DOI:
10.1073/pnas.1710470114
Publication Date:
2017-08-23T00:35:14Z
AUTHORS (11)
ABSTRACT
SignificanceThe human placenta is a dynamic and cellular heterogeneous organ, which is critical in fetomaternal homeostasis and the development of preeclampsia. Previous work has shown that placenta-derived cell-free RNA increases during pregnancy. We applied large-scale microfluidic single-cell transcriptomic technology to comprehensively characterize cellular heterogeneity of the human placentas and identified multiple placental cell-type–specific gene signatures. Analysis of the cellular signature expression in maternal plasma enabled noninvasive delineation of the cellular dynamics of the placenta during pregnancy and the elucidation of extravillous trophoblastic dysfunction in early preeclampsia.
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