Molecular clutch drives cell response to surface viscosity

• DOI: 10.1073/pnas.1710653115
• Publication Date: 2018-01-22T20:07:12Z
• Authors: Mark Bennett; Marco Cantini; Julien Reboud; Jonathan M. Cooper; Pere Roca-Cusachs; Manuel Salmeron-S...
• Keywords: 0301 basic medicine; Molecular biology; Surface Properties; Lipid Bilayers; Cell Cycle Proteins; Microscopy, Atomic Force; Cell Line; Myoblasts; Mice; 03 medical and health sciences; Animals; surface viscosity; Cell Shape; mechanotransduction; Biologia molecular; Teixits (Histologia); Adaptor Proteins, Signal Transducing; Microscopy; Focal Adhesions; Viscosity; Signal Transducing; Adaptor Proteins; Atomic Force; YAP-Signaling Proteins; Phosphoproteins; Actins; matrix rigidity; Extracellular Matrix; Fibronectins; cell differentiation; Tissues; Focal Adhesion Kinase 1; Physical Sciences; Viscositat; Phosphatidylcholines; molecular clutch; Oligopeptides

Cell response to matrix rigidity has been explained by the mechanical properties of actin-talin-integrin-fibronectin clutch. Here molecular clutch model is extended account for cell interactions with purely viscous surfaces (i.e., without an elastic component). Supported lipid bilayers present idealized and controllable system through which study this concept. Using lipids different diffusion coefficients, mobility surface viscosity) presented ligands (in case RGD) was altered order magnitude. size cytoskeletal organization were proportional viscosity. Furthermore, there a higher number focal adhesions phosphorylation FAK on less-mobile (more-viscous) surfaces. Actin retrograde flow, indicator force exerted surfaces, also seen be faster more mobile This consequential effects downstream molecules; mechanosensitive YAP protein localized nucleus differentiation myoblast cells enhanced behavior within framework model, lower viscosity leading low loading rate, preventing exposure proteins, causing rate exposing these sites, activating pathways. Consequently, understanding how (regardless stiffness) influences adds further tool engineer materials that control behavior.