Human N -acetylglucosaminyltransferase II substrate recognition uses a modular architecture that includes a convergent exosite

• DOI: 10.1073/pnas.1716988115
• Publication Date: 2018-04-17T15:01:29Z
• Authors: Renuka Kadirvelraj; Jeong-Yeh Yang; Justin H. Sanders; Lin Liu; Annapoorani Ramiah; Pradeep Kumar Pra...; Geert-Jan Boons; Zachary A. Wood; Kelley W. Moremen
• Keywords: Transferase

Significance Cell-surface and secreted glycoproteins are initially synthesized glycosylated in the endoplasmic reticulum. Glycan structures trimmed remodeled as they transit secretory pathway, resulting multi-branched complex-type structures. The enzymes that remodel these have precise linkage branch specificities, with product of one reaction being specifically recognized substrate for following reaction. These reactions include N -acetylglucosaminyltransferase II (MGAT2), an enzyme initiates complex extension by recognition its glycan substrate. structural basis MGAT2 is subject present study. Structures MGAT2-substrate complexes reveal both modular convergent mechanisms selective catalysis provide a generalized model template-based synthesis glycosyltransferases.