Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy

• DOI: 10.1073/pnas.1813044116
• Publication Date: 2019-03-15T00:34:48Z
• Authors: Mian Wei; Lingyan Shi; Yihui Shen; Zhilun Zhao; Asja Guzman; Laura J. Kaufman; Lu Wei; Wei Min
• Keywords: Fluorescence-lifetime imaging microscopy; Chemical Imaging

Significance Cells form structures and perform functions through intricate 3D tissue organizations. However, due to scattering, coherent Raman microscopy—a powerful method complementary fluorescence imaging—suffers from limited imaging depth in tissues. Here, we develop a volumetric chemical with greater than 10-fold increase. We formulate Raman-tailored tissue-clearing recipe combine it advanced microscopies. Equipped the toolbox of analyses, elucidate complex structures, compositions, metabolic dynamics diverse tissues including lipid synthesis throughout tumor spheroids, networks axons, vasculatures, cell bodies brain regions, as well heterogeneous metabolism.