Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships

• DOI: 10.1073/pnas.1813957116
• Publication Date: 2019-01-18T03:05:56Z
• Authors: Patrice S. Albert; Tao Zhang; Kassandra Semrau; Jean-Marie Rouillard; Yu-Hsin Kao; Chung-Ju Rachel Wang; Tatiana V. Danilova; Jiming Jiang; James A. Birchler
• Keywords: Cell Nucleus; Chromosome Aberrations; Gene Rearrangement; 2. Zero hunger; 0303 health sciences; Transcription, Genetic; Chromosomal aberrations; Karyotype; Oligonucleotides; 610; B chromosome; Chromosomes, Plant; Chromosome Painting; 03 medical and health sciences; Chromosome painting; PNAS Plus; Oligo-FISH; DNA Probes; Genome, Plant; Metaphase

Significance Whole-chromosome oligo-FISH paints using synthetic oligonucleotide libraries that can be amplified and labeled were generated for all 10 chromosomes of maize, facilitating chromosome studies with high sensitivity specificity genetically diverse lines. Applications include visualization simple or complex chromosomal aberrations, establishment domains, illustration mitotic meiotic behavior, providing insights into relationships.