The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis

• DOI: 10.1073/pnas.1819077116
• Publication Date: 2019-06-11T00:26:35Z
• Authors: J. F. McKenna; D. J. Rolfe; S. E. D. Webb; A. F. Tolmie; S. W. Botchway; M. L. Martin-Fern...; C. Hawes; J. Runions
• Keywords: Dynamics

Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these to function effectively there needs be structure within the PM allowing, for example, same signaling cascade spatially organized. Here we demonstrate that with divergent functions located clusters differing size membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals move at different rates membrane. Actin microtubule cytoskeletons appear significantly regulate mobility one (the pathogen receptor FLS2) further cell wall is critical regulation cluster by quantifying single dynamics key roles morphogenesis (PIN3) perception (FLS2). We propose a model which cytoskeleton pivotal protein dynamics, thereby contributing formation functionality nanodomains.