Dominant-negative inhibition of prion replication in transgenic mice
Immunoassay
0303 health sciences
Polymorphism, Genetic
PrPSc Proteins
Prions
Protein Conformation
Immunoblotting
Brain
Mice, Transgenic
3. Good health
Mice
03 medical and health sciences
Calibration
Animals
Humans
Protein Isoforms
Transgenes
Genes, Dominant
Scrapie
DOI:
10.1073/pnas.182425299
Publication Date:
2002-10-01T16:58:07Z
AUTHORS (9)
ABSTRACT
Our discovery of dominant-negative inhibition of prion formation in cultured cells provided an explanation for the resistance of some sheep to scrapie and humans to Creutzfeldt–Jakob disease. To determine whether dominant-negative inhibition occurs
in vivo
, we produced transgenic (Tg) mice expressing prion protein (PrP) with either the Q167R or Q218K mutation alone or in combination with wild-type (wt) PrP. Tg(MoPrP,Q167R)
Prnp
0/0
mice expressing mutant PrP at levels equal to non-Tg mice remained healthy for >550 days, indicating that inoculation with prions did not cause disease. Immunoblots of brain homogenates and histologic analysis did not reveal abnormalities. Tg(MoPrP,Q167R)
Prnp
+/+
mice expressing both mutant and wt PrP did not exhibit neurologic dysfunction, but their brains revealed low levels of the PrP pathogenic isoform (PrP
Sc
), and sections showed numerous vacuoles and severe astrocytic gliosis at 300 days after inoculation. Both Tg(MoPrP,Q218K)
Prnp
0/0
and Tg(MoPrP,Q218K)
Prnp
+/+
mice expressing high levels of the transgene product remained healthy for >300 days after inoculation. Neither PrP
Sc
nor neuropathologic changes were found. Our studies demonstrate that although dominant-negative inhibition of wt PrP
Sc
formation occurs, expression of the dominant-negative PrP at the same level as wt PrP does not prevent prion formation completely. However, expression of dominant-negative PrP alone had no deleterious effects on the mice and did not support prion propagation.
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