Dynamic secretome of bone marrow-derived stromal cells reveals a cardioprotective biochemical cocktail

• DOI: 10.1073/pnas.1902598116
• Publication Date: 2019-06-25T21:10:17Z
• Authors: Kshitiz; David D. Ellison; Yasir Suhail; Junaid Afzal; Laura Woo; Onur Kilic; Jeffrey Spees; Andre Levchenko
• Keywords: Cells; Myocardial Infarction; Neovascularization, Physiologic; Bone Marrow Cells; secretion dynamics; paracrine signaling; Ligands; 03 medical and health sciences; Bone Marrow; Paracrine Communication; Animals; Humans; AC133 Antigen; Physiologic; Neovascularization; Cells, Cultured; Inflammation; 0303 health sciences; Cultured; Cell Differentiation; Mesenchymal Stem Cells; cell–cell communication; secretome; Oxidative Stress; paracrine dynamics

Significance Intercellular communication between cells in tissues is frequently mediated by secreted factors, with rates of secretion frequently varying over time. Stem cells can be recruited and contribute to the reparative process in diverse tissues. How this versatile cytoprotective effect is achieved is not well understood. Here we describe a simple-to-use platform to estimate the dynamics of cellular paracrine signaling in response to a wide range of physiologically relevant perturbations. When injured cardiomyocytes were presented with a cocktail matching the dynamics and dosage of stromal cell-derived secreted factors, we found an improved survival response, suggesting that secretory dynamics can be a key component of therapeutic recombinant cocktails for tissue repair.