A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae
STED microscopy
Organelle
Live cell imaging
Fluorescence-lifetime imaging microscopy
DOI:
10.1073/pnas.1905924116
Publication Date:
2019-07-23T20:49:14Z
AUTHORS (7)
ABSTRACT
Significance Stimulated emission depletion (STED) microscopy is one of the most appealing tools to visualize nanoscale cellular structures and dynamics in living cells. However, its practical utility significantly limited by rapid photobleaching fluorescent dyes under ultrastrong laser. In this context, superphotostable probes, which enable repeated recording STED images, are crucial chemical extend application live-cell imaging. Herein, we report a developed probe, MitoPB Yellow, has characters outstanding photostability, long fluorescence lifetime, mitochondrial inner membrane selectivity for staining. combination with time-gated-STED microscopy, inner-membrane can be impressively visualized
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