A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae

STED microscopy Organelle Live cell imaging Fluorescence-lifetime imaging microscopy
DOI: 10.1073/pnas.1905924116 Publication Date: 2019-07-23T20:49:14Z
ABSTRACT
Significance Stimulated emission depletion (STED) microscopy is one of the most appealing tools to visualize nanoscale cellular structures and dynamics in living cells. However, its practical utility significantly limited by rapid photobleaching fluorescent dyes under ultrastrong laser. In this context, superphotostable probes, which enable repeated recording STED images, are crucial chemical extend application live-cell imaging. Herein, we report a developed probe, MitoPB Yellow, has characters outstanding photostability, long fluorescence lifetime, mitochondrial inner membrane selectivity for staining. combination with time-gated-STED microscopy, inner-membrane can be impressively visualized
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