A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae

• DOI: 10.1073/pnas.1905924116
• Publication Date: 2019-07-23T20:49:14Z
• Authors: Chenguang Wang; Masayasu Taki; Yoshikatsu Sato; Yasushi Tamura; Hideyuki Yaginuma; Yasushi Okada; Shigehiro Yamaguchi
• Keywords: STED microscopy; Organelle; Live cell imaging; Fluorescence-lifetime imaging microscopy

Significance Stimulated emission depletion (STED) microscopy is one of the most appealing tools to visualize nanoscale cellular structures and dynamics in living cells. However, its practical utility significantly limited by rapid photobleaching fluorescent dyes under ultrastrong laser. In this context, superphotostable probes, which enable repeated recording STED images, are crucial chemical extend application live-cell imaging. Herein, we report a developed probe, MitoPB Yellow, has characters outstanding photostability, long fluorescence lifetime, mitochondrial inner membrane selectivity for staining. combination with time-gated-STED microscopy, inner-membrane can be impressively visualized