Methodology and theoretical basis of forward genetic screening for sleep/wakefulness in mice

• DOI: 10.1073/pnas.1906774116
• Publication Date: 2019-07-23T20:49:08Z
• Authors: Chika Miyoshi; Staci J. Kim; Takahiro Ezaki; Aya Ikkyu; Noriko Hotta-Hira...; Satomi Kanno; Miyo Kakizaki; Mana Yamada; Shigeharu Wakana; Masashi Yanagisawa; Hiromasa Funato
• Keywords: Sleep

Significance The network of genes and molecules that govern sleep wakefulness remains largely unknown. Forward genetics is a powerful approach to elucidate important biological phenomena cannot be predicted from the function known genes. We established large-scale screening system using EEG/EMG-based sleep/wake monitoring with reliable parameters. search for novel has succeeded in identifying several regulatory By furthering study, more comprehensive picture pathways sleep/wakefulness will become clear.