Functional cooperation of the glycine synthase-reductase and Wood–Ljungdahl pathways for autotrophic growth of Clostridium drakei

• DOI: 10.1073/pnas.1912289117
• Publication Date: 2020-03-14T00:05:52Z
• Authors: Yoseb Song; Jin Soo Lee; Jongoh Shin; Gyu Min Lee; Sangrak Jin; Seulgi Kang; Jung-Kul Lee; Dong Rip Kim; Eun Yeol Lee; Sun Chang Kim; Suhyung Cho; Donghyuk Kim; Byung-Kwan Cho
• Keywords: Metabolic pathway; Autotroph; Glutamate synthase; Glutamine amidotransferase

Among CO 2 -fixing metabolic pathways in nature, the linear Wood–Ljungdahl pathway (WLP) phylogenetically diverse acetate-forming acetogens comprises most energetically efficient pathway, requires least number of reactions, and converts to formate then into acetyl-CoA. Despite two genes encoding glycine synthase being well-conserved WLP gene clusters, functional role under autotrophic growth conditions has remained uncertain. Here, using reconstructed genome-scale model i SL771 based on completed genome sequence, transcriptomics, 13 C isotope-based metabolite-tracing experiments, biochemical assays, heterologous expression another acetogen, we discovered that are functionally interconnected fix , subsequently converting acetyl-CoA, acetyl-phosphate, serine. Moreover, cooperation enhances consumption cellular rates via bypassing reducing power required reactions for metabolism during acetogens.