Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen

• DOI: 10.1073/pnas.191380898
• Publication Date: 2002-07-26T14:34:10Z
• Authors: Oscar Bruña-Romero; Gloria González-A...; Julius C. R. Hafalla; Moriya Tsuji; Ruth S. Nussenzweig
• Keywords: Circumsporozoite protein; Priming (agriculture); Plasmodium yoelii; Modified vaccinia Ankara

We report that complete protection against malaria and total inhibition of liver stage development parasitemia was obtained in 100% BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein Plasmodium yoelii (AdPyCS), followed by booster an attenuated vaccinia virus, same antigen, VacPyCS. found increased levels activated CS-specific CD8(+) CD4(+) T cells, higher anti-sporozoite antibody titers, greater these mice, when time between priming boosting two viral vectors extended from 2 to 8 or more weeks. Most importantly, using this immunization regimen, immunized be long-lasting, namely resistance infection all animals 3 1/2 months after priming. These results indicate AdPyCS generates highly effective memory B cells can recalled long