The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity

• DOI: 10.1073/pnas.1920109117
• Publication Date: 2020-06-16T00:26:30Z
• Authors: Dominic T. Castanzo; Benjamin LaFrance; Andreas Martin
• Keywords: Random hexamer; AAA proteins; Translocase

Significance The AAA+ ATPase Msp1 has previously been shown to function in the quality control of tail-anchored proteins at mitochondrial and peroxisomal membranes, yet its detailed mechanisms substrate processing have remained unknown. Using scaffolds stabilize functional hexamer domains various model derived from Msp1’s endogenous Pex15, we show that is a promiscuous protein translocase mechanically unfolds pulls substrates through central pore an N-to-C or C-to-N terminal direction. Pex3 modulates activity peroxisome by directly interacting with inhibiting threading.