Cryo-EM analysis of a membrane protein embedded in the liposome

• DOI: 10.1073/pnas.2009385117
• Publication Date: 2020-07-18T00:06:56Z
• Authors: Xia Yao; Xiao Fan; Nieng Yan
• Keywords: Models, Molecular; 0301 basic medicine; 03 medical and health sciences; Protein Conformation; Escherichia coli Proteins; Cell Membrane; Cryoelectron Microscopy; Liposomes; Escherichia coli; Membrane Proteins; Multidrug Resistance-Associated Proteins

Significance Membrane proteins (MPs), encoded by ∼30% of the coding genes, play vital roles in numerous physiological processes. MPs are targets more than half FDA-approved drugs. High-resolution structural studies functional membrane under near-physiological conditions required to provide an in-depth mechanistic understanding and facilitate drug discovery. To embed into liposomes represents a strategy mimic native conditions. Here we present convenient workflow for cryo-EM analysis liposome-embedded using prototypal protein AcrB. Our method sets foundation future investigation presence electrochemical gradients interdependence integral or peripheral various properties.