Intercellular communication induces glycolytic synchronization waves between individually oscillating cells

• DOI: 10.1073/pnas.2010075118
• Publication Date: 2021-02-01T22:35:40Z
• Authors: Martin Mojica-Ben...; David D. van Niekerk; Mite Mijalkov; Jacky L. Snoep; Bernhard Mehlig; Giovanni Volpe; Mattias Goksör; Caroline B. Adiels
• Keywords: 0301 basic medicine; 0303 health sciences; Time Factors; Glycolytic oscillations; Microfluidics; FOS: Physical sciences; Cell Communication; Saccharomyces cerevisiae; Cell-cell communication; Condensed Matter - Soft Condensed Matter; 03 medical and health sciences; Biological Physics (physics.bio-ph); FOS: Biological sciences; Physical Sciences; Cell Behavior (q-bio.CB); Quantitative Biology - Cell Behavior; Soft Condensed Matter (cond-mat.soft); Computer Simulation; Physics - Biological Physics; Extracellular Space; Glycolysis; Synchronization waves

Metabolic oscillations in single cells underlie the mechanisms behind cell synchronization and cell-cell communication. For example, glycolytic mediated by biochemical communication between may synchronize pulsatile insulin secretion pancreatic tissue, a link anomalies type-2 diabetes has been hypotesized. Cultures of yeast have provided an ideal model system to study propagation waves large populations. However, mechanism which occurs at individual level overcome local chemical concentrations heterogenic biological clocks, is still open question because experimental limitations sensitive specific handling cells. Here, we show how coupling intercellular diffusion with phase regulation oscillating induce waves. We directly measure single-cell metabolic responses from microfluidic environment characterize discretized using graph theory. corroborate our findings simulations based on kinetic detailed for These can provide insight into roles cellular play biomedical applications, such as level.