TCRα reporter mice reveal contribution of dual TCRα expression to T cell repertoire and function

• DOI: 10.1073/pnas.2013188117
• Publication Date: 2020-12-07T21:55:48Z
• Authors: Letitia Yang; Burhan Jama; Huawei Wang; Lara Labarta-Bajo; Elina I. Zúñiga; Gerald P. Morris
• Keywords: CD4-Positive T-Lymphocytes; Male; 0301 basic medicine; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocytes; Green Fluorescent Proteins; Gene Expression; Mice, Transgenic; CD8-Positive T-Lymphocytes; Lymphocytic Choriomeningitis; Inbred C57BL; CD5 Antigens; Transgenic; Mice; 03 medical and health sciences; Receptors; Chlorocebus aethiops; 2.1 Biological and endogenous factors; Animals; Lymphocytic choriomeningitis virus; Aetiology; LCMV; Vero Cells; alpha-beta; 0303 health sciences; Thymocytes; Prevention; Inflammatory and immune system; T cell; T-Cell; 3. Good health; Mice, Inbred C57BL; Infectious Diseases; Emerging Infectious Diseases; Genes; 13. Climate action; Antigen; Female; T cell receptor; T-Cell Receptor alpha; Immunologic Memory; TCR; Genes, T-Cell Receptor alpha

Significance This study utilizes novel transgenic reporter mice to definitively evaluate T cells coexpressing two cell antigen receptors. These experiments demonstrate that lymphocytes naturally different receptors are significantly more abundant than previous estimates and these have a previously unappreciated role in physiologic immune responses.