Real-time measurements of aminoglycoside effects on protein synthesis in live cells

• DOI: 10.1073/pnas.2013315118
• Publication Date: 2021-02-22T21:27:20Z
• Authors: Javier Aguirre Ri...; Jimmy Larsson; Ivan L. Volkov; A. Carolin Seefeldt; Suparna Sanyal; Magnus Johansson
• Keywords: translation; Biochemistry; antibiotics; 03 medical and health sciences; RNA, Transfer; Escherichia coli; Biokemi; Molecular Biology; tRNA; Protein Synthesis Inhibitors; 0303 health sciences; Molekylärbiologi; single-molecule tracking; Biochemistry and Molecular Biology; Biological Sciences; Anti-Bacterial Agents; Molecular Imaging; 3. Good health; Aminoglycosides; Microscopy, Fluorescence; Protein Biosynthesis; microscopy; Single-Cell Analysis; Ribosomes; superresolution; Biokemi och molekylärbiologi

Significance To address the public health challenges caused by spread of antibiotic resistance, it is critical to enhance our understanding mechanisms action these compounds. In present study, we use superresolved single-molecule tracking techniques, investigate effect three aminoglycoside drugs on protein synthesis kinetics directly inside live bacterial cells. Our results imply that do not completely inhibit synthesis, but only make slower. Hence, bactericidal likely due a disturbed, rather than inhibited, process.