Runx1 and Runx3 drive progenitor to T-lineage transcriptome conversion in mouse T cell commitment via dynamic genomic site switching

• DOI: 10.1073/pnas.2019655118
• Publication Date: 2021-01-21T21:57:57Z
• Authors: Boyoung Shin; Hiroyuki Hosokawa; Maile Romero-Wolf; Wen Zhou; Kaori Masuhara; Victoria R. Tobin; Ditsa Levanon; Yoram Groner; Ellen V. Rothenberg
• Keywords: RUNX1; Cell fate determination

Significance T-lineage specification requires waves of gene network changes to terminate multipotency and establish T identity. Circumstantial evidence has suggested roles for Runt domain-related (Runx) factors in these regulatory events; Runx1 is strongly expressed, but knockouts have previously shown little impact during stages around commitment. We show that Runx3 function together drive commitment, binding the same sites. Double block both activation genes repression progenitor genes. Contrasting with stable activity Runx proteins throughout stages, preferentially regulate target sharply change expression this reflects pronounced, global redistributions choices across genome before after