Droplet-based mRNA sequencing of fixed and permeabilized cells by CLInt-seq allows for antigen-specific TCR cloning

• DOI: 10.1073/pnas.2021190118
• Publication Date: 2021-01-12T21:10:49Z
• Authors: Pavlo A. Nesterenko; Jami McLaughlin; Donghui Cheng; Nathanael J. Bang...; Giselle Burton Sojo; Christopher S. Seet; Yu Qin; Zhiyuan Mao; Matthew B. Obusan; John W. Phillips; Owen N. Witte
• Keywords: Single cell sequencing; Cloning (programming)

Significance T cell receptors (TCRs) surveil cellular environment by recognizing peptides presented the major histocompatibility complex. TCR sequencing allows for understanding scope of reactivity in health and disease. Specific clones can be used as therapeutics cancer autoimmune We present a technique that based on intracellular signaling molecules, such cytokines transcription factors. The core concept is highly generalizable should applicable to global gene expression analysis where marker-based isolation required.