Molecular basis of the dual role of the Mlh1-Mlh3 endonuclease in MMR and in meiotic crossover formation

• DOI: 10.1073/pnas.2022704118
• Publication Date: 2021-06-04T20:23:23Z
• Authors: Jingqi Dai; Aurore Sanchez; Céline Adam; Lepakshi Ranjha; Giordano Reginato; Pierre Chervy; Carine Tellier-Le...; Jessica Andreani; Raphaël Guérois; Virginie Ropars; Marie-Hélène Le Du; Laurent Maloisel; Emmanuelle Martini; Pierre Legrand; Aurélien Thureau; Petr Cejka; Valérie Borde; Jean-Baptiste Cha...
• Keywords: Models, Molecular; 0303 health sciences; Binding Sites; Saccharomyces cerevisiae Proteins; DNA Repair; [SDV]Life Sciences [q-bio]; Recombinational DNA Repair; Saccharomyces cerevisiae; Endonucleases; DNA Mismatch Repair; DNA-Binding Proteins; Meiosis; 03 medical and health sciences; MutL Proteins; MutL Protein Homolog 1

Significance During meiosis, programmed chromosome breakage and subsequent double-stranded DNA (dsDNA) break repair help ensure correct segregation promote genetic diversity of the progeny. In budding yeast, which utilizes meiotic recombination pathways conserved in mice humans, majority crossovers are initiated through formation a Holliday junction, requires endonuclease activity Mlh1-Mlh3 mismatch factor to be resolved exclusively into crossover product. Here, we combined structural biology, biochemical, analyses compare structure functions with main Mlh1-Pms1. We characterize differences around their respective sites. also mutants associated condensation filament heterodimer.