Protein interaction landscapes revealed by advanced in vivo cross-linking–mass spectrometry

• DOI: 10.1073/pnas.2023360118
• Publication Date: 2021-08-04T20:30:14Z
• Authors: Andrew Wheat; Clinton Yu; Xiaorong Wang; Anthony M. Burke; Ilan E. Chemmama; Robyn M. Kaake; Peter Baker; Scott D. Rychnovsky; Jing Yang; Lan Huang
• Keywords: Interactome; Proteome

Significance Blueprints of in-cell protein interaction landscapes are essential for our understanding cellular structures and functions, which have been challenging to study at the systems level. Cross-linking–mass spectrometry (XL-MS) represents a high-throughput method global profiling networks can determine identity connectivity native PPIs simultaneously without cell engineering. While in vivo XL-MS experiments feasible, in-depth analyses remain difficult due technical limitations on sample preparation. Here, we developed new Alkyne-A-DSBSO–based platform that enabled us obtain most comprehensive PPI maps cells. This approach be adopted proteome-wide studies any organisms origins, thus advancing interactome biology beyond proteome abundance.