Drugs repurposed for COVID-19 by virtual screening of 6,218 drugs and cell-based assay

0301 basic medicine 0303 health sciences Alanine Drug Evaluation, Preclinical Drug Repositioning Drug Synergism Biological Sciences Antiviral Agents Adenosine Monophosphate COVID-19 Drug Treatment 3. Good health User-Computer Interface 03 medical and health sciences Chlorocebus aethiops Animals Humans Vero Cells
DOI: 10.1073/pnas.2024302118 Publication Date: 2021-07-07T19:32:35Z
ABSTRACT
Significance Recent spread of SARS-CoV-2 has sparked significant health concerns of emerging infectious viruses. Drug repurposing is a tangible strategy for developing antiviral agents within a short period. In general, drug repurposing starts with virtual screening of approved drugs employing docking simulations. However, the actual hit rate is low, and most of the predicted compounds are false positives. To tackle the challenges, we report advanced virtual screening with pre- and postdocking pharmacophore filtering of 6,218 drugs for COVID-19. Notably, 7 out of 38 compounds showed efficacies in inhibiting SARS-CoV-2 in Vero cells. Three of these were also found to inhibit SARS-CoV-2 in human Calu-3 cells. Furthermore, three drug combinations showed strong synergistic effects in SARS-CoV-2 inhibition at their clinically achievable concentrations.
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