Structural changes in bacteriophage T7 upon receptor-induced genome ejection

• DOI: 10.1073/pnas.2102003118
• Publication Date: 2021-09-14T17:58:14Z
• Authors: Wenyuan Chen; Hao Xiao; Li Wang; Xurong Wang; Zhixue Tan; Zhen Han; Xiaowu Li; Fan Yang; Zhonghua Liu; Jingdong Song; Hongrong Liu; Lingpeng Cheng
• Keywords: Bacterial virus

Significance Many double-stranded DNA (dsDNA) viruses, including the tailed dsDNA bacteriophages and herpesviruses, use molecular machines at a unique vertex of capsid for genome delivery. Bacteriophage T7 provides tractable model study viruses. Here, we report structure mature bacteriophage T7, as well structures full empty particles in complex with their cell receptor lipopolysaccharide. The symmetry-mismatch vertex, internal ejection proteins, have been resolved near-atomic resolutions our structures. different stages reveal series structural rearrangements after viral