Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW
Proteomics
0301 basic medicine
0303 health sciences
Chemotactic Factors
Chemotaxis
Iron
Catechols
Biological Sciences
ddc:
03 medical and health sciences
Catecholamines
Bacterial Proteins
Molecular Probes
Protein Binding
Signal Transduction
Vibrio
DOI:
10.1073/pnas.2118227119
Publication Date:
2022-03-02T16:33:22Z
AUTHORS (8)
ABSTRACT
Significance
Host-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogen
Vibrio campbellii
. Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.
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CITATIONS (11)
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