Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW

Proteomics 0301 basic medicine 0303 health sciences Chemotactic Factors Chemotaxis Iron Catechols Biological Sciences ddc: 03 medical and health sciences Catecholamines Bacterial Proteins Molecular Probes Protein Binding Signal Transduction Vibrio
DOI: 10.1073/pnas.2118227119 Publication Date: 2022-03-02T16:33:22Z
ABSTRACT
Significance Host-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogen Vibrio campbellii . Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.
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