Interplay between Asters/GRAMD1s and phosphatidylserine in intermembrane transport of LDL cholesterol

• DOI: 10.1073/pnas.2120411119
• Publication Date: 2022-01-06T21:05:19Z
• Authors: Michael N. Trinh; Michael S. Brown; Joachim Seemann; Gonçalo Vale; Jeffrey G. McDonald; Joseph L. Goldstein; Feiran Lu
• Keywords: Cell Membrane; Membrane Proteins; Biological Transport; CHO Cells; Cholesterol, LDL; Phosphatidylserines; Biological Sciences; Endoplasmic Reticulum; 16. Peace & justice; Endocytosis; Cholesterol; Cricetulus; Cricetinae; Animals; Cholesterol Esters; Lysosomes

Significance Cholesterol constitutes 50% of lipids in the plasma membrane (PM) animal cells. Sensors endoplasmic reticulum (ER) maintain this level by adjusting cholesterol uptake, synthesis, and storage. Uptake is mediated LDL receptors, which deliver cholesterol-carrying to lysosomes from moves PM then ER. We report PM-to-ER transport requires cholesterol-binding Aster proteins anchored ER phosphatidylserine embedded PM. Asters are known bind phosphatidylserine, accounts for part requirement. However, current data suggest an additional requirement independent Asters. These advance our knowledge homeostasis, a control mechanism essential cell growth survival.