Nano-positioning and tubulin conformation contribute to axonal transport regulation of mitochondria along microtubules

• DOI: 10.1073/pnas.2203499119
• Publication Date: 2022-11-02T18:50:06Z
• Authors: Valérie Van Steen...; Flavie Lavoie-Car...; Youcef Kazwiny; Marianna Decet; Tobie Martens; Patrik Verstreken; Werend Boesmans; Paul De Koninck; Pieter Vanden Berghe
• Keywords: Kinesin; Axoplasmic transport; Organelle

Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning regulated by intracellular transport mechanisms. Controlled bulky mitochondria especially important in polarized cells such as neurons that rely on these to locally produce energy buffer calcium. Mitochondrial requires depends microtubules fill much the available axonal space. How mitochondrial affected their position within microtubule bundles not known. Here, we found anterograde transport, driven kinesin motors, susceptible molecular conformation tubulin both vitro vivo. Anterograde velocities negatively correlate with density elongated dimers like guanosine triphosphate (GTP)-tubulin. The impact primarily where a mitochondrion positioned, either or at rim bundle. Increasing levels lowers number motile bundle increases speed rim. We demonstrate increased velocity consisting add dynamics. Our work indicates contributes regulation motility local along axons.