Gaussian curvature–driven direction of cell fate toward osteogenesis with triply periodic minimal surface scaffolds

• DOI: 10.1073/pnas.2206684119
• Publication Date: 2022-10-03T19:17:41Z
• Authors: Yuhe Yang; Tianpeng Xu; Ho-Pan Bei; Lei Zhang; Chak-Yin Tang; Ming Zhang; Chenjie Xu; Liming Bian; Kelvin Wai-Kwok Y...; Jerry Ying Hsi Fuh; Xin Zhao
• Keywords: Interconnectivity; Fibrocartilage

Leaf photosynthesis, coral mineralization, and trabecular bone growth depend on triply periodic minimal surfaces (TPMSs) with hyperboloidal structure every surface point varying Gaussian curvatures. However, translation of this into tissue-engineered grafts is challenging. This article reports the design fabrication high-resolution three-dimensional TPMS scaffolds embodying biomimicking topography different curvatures, composed body inherent β-tricalcium phosphate, by stereolithography-based printing sintering. The show high porosity interconnectivity. Notably, compared conventional scaffolds, they can reduce stress concentration, leading to increased mechanical strength. They are also found support attachment, proliferation, osteogenic differentiation, angiogenic paracrine function human mesenchymal stem cells (hMSCs). Through transcriptomic analysis, we theorize that hyperboloid induces cytoskeleton reorganization hMSCs, expressing elongated morphology convex direction strengthening cytoskeletal contraction. clinical therapeutic efficacy assessed rabbit femur defect mouse subcutaneous implantation models demonstrate augment new formation neovascularization. In comparison our successfully guide cell fate toward osteogenesis through cell-level directional curvatures drastic yet quantifiable improvements in regeneration.