Endoplasmic reticulum–bound ANAC013 factor is cleaved by RHOMBOID-LIKE 2 during the initial response to hypoxia in Arabidopsis thaliana

• DOI: 10.1073/pnas.2221308120
• Publication Date: 2023-03-10T19:05:58Z
• Authors: Emese Eysholdt-De...; Tilo Renziehausen; Stephanie Frings; Stephanie Frohn; Kira von Bongartz; Clara P. Igisch; Justina Mann; Lisa Häger; Julia Macholl; David Leisse; Niels Hoffmann; Katharina Winkels; Pia Wanner; Jonas De Backer; Xiaopeng Luo; Margret Sauter; Inge De Clercq; Joost T. van Dongen; Jos H. M. Schippers; Romy R. Schmidt-S...
• Keywords: Hypoxia

Aerobic reactions are essential to sustain plant growth and development. Impaired oxygen availability due excessive water availability, e.g., during waterlogging or flooding, reduces productivity survival. Consequently, plants monitor adjust metabolism accordingly. Despite the identification of central components in hypoxia adaptation recent years, molecular pathways involved very early activation low-oxygen responses insufficiently understood. Here, we characterized three endoplasmic reticulum (ER)-anchored Arabidopsis ANAC transcription factors, namely ANAC013, ANAC016, ANAC017, which bind promoters a subset core genes (HCGs) activate their expression. However, only ANAC013 translocates nucleus at onset hypoxia, i.e., after 1.5 h stress. Upon nuclear associates with multiple HCGs. Mechanistically, identified residues transmembrane domain be for factor release from ER, provide evidence that RHOMBOID-LIKE 2 (RBL2) protease mediates under hypoxia. Release by RBL2 also occurs upon mitochondrial dysfunction. Consistently, like knockdown lines, rbl knockout mutants exhibit impaired tolerance. Taken together, uncovered an ER-localized ANAC013-RBL2 module, is active initial phase enable fast transcriptional reprogramming.