The Drosophila blood–brain barrier regulates sleep via Moody G protein-coupled receptor signaling

• DOI: 10.1073/pnas.2309331120
• Publication Date: 2023-10-13T17:47:26Z
• Authors: Sofia Axelrod; Xiaoling Li; Yingwo Sun; Samantha Lincoln; Andrea Terceros; Jenna O’Neil; Zikun Wang; Andrew Nguyen; Aabha Vora; Carmen Spicer; Benjamin Shapiro; Michael W. Young
• Keywords: Sleep; Homeostasis

Sleep is vital for most animals, yet its mechanism and function remain unclear. We found that permeability of the BBB (blood–brain barrier)—the organ required maintenance homeostatic levels nutrients, ions, other molecules in brain—is modulated by sleep deprivation (SD) can cell-autonomously effect changes. observed increased known mutants as well acutely sleep-deprived animals. In addition to molecular tracers, SD–induced changes also penetration drugs used treatment brain pathologies. After chronic/genetic or acute SD, rebound administration sleeping aid gaboxadol normalized permeability, showing SD effects on are reversible. Along with RNA master regulator moody sleep. Conversely, altering alone through glia-specific modulation moody, gαo, loco, lachesin , neuroglian —each a well-studied function—was sufficient induce robust phenotypes. These studies demonstrate tight link between indicate unique role regulation