An architecturally rational hemostat for rapid stopping of massive bleeding on anticoagulation therapy

• DOI: 10.1073/pnas.2316170121
• Publication Date: 2024-01-22T20:07:01Z
• Authors: Vivian K. Lee; Taewoo Lee; Amrit Ghosh; Tanmoy Saha; Manish V. Bais; Kala Kumar Bharani; Milan Chag; Keyur Parikh; Parloop Bhatt; Bumseok Namgung; Geethapriya Venka...; Animesh Agrawal; Kiran Sonaje; Leo Mavely; Shiladitya Sengupta; Raghunath Anant M...; Hae Lin Jang
• Keywords: Hemostat

Hemostatic devices are critical for managing emergent severe bleeding. With the increased use of anticoagulant therapy, there is a need next-generation hemostats. We rationalized that hemostat with an architecture designed to increase contact blood, and engineered from material activates distinct undrugged coagulation pathway can address emerging need. Inspired by lung alveolar architecture, here, we describe engineering single-phase chitosan tortuous spherical microporous design enables rapid blood absorption concentrated platelets fibrin microthrombi in localized regions, phenomenon less observed other classical hemostats without structural optimization. The interaction between components porous was further amplified based on charged surface chitosan. Contrary dogma does not directly affect physiological clotting mechanism, induced via direct activation platelet Toll-like receptor 2. Our effectively stopped bleeding murine liver wounds, swine carotid artery injuries, human radial puncture site within few minutes significantly reduced loss, even under treatment. integration principles understanding molecular mechanisms lead improved functions medical needs.