Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5

• DOI: 10.1073/pnas.2317408121
• Publication Date: 2024-01-29T20:00:21Z
• Authors: Yan Yan; Haofei Luo; Yuwei Qin; Tingting Yan; Jinbu Jia; Yifeng Hou; Zhijian Liu; Jixian Zhai; Yanping Long; Xian Deng; Xiaofeng Cao
• Keywords: Splicing factor; Photomorphogenesis

Light plays a central role in plant growth and development, providing an energy source governing various aspects of morphology. Previous study showed that many polyadenylated full-length RNA molecules within the nucleus contain unspliced introns (post-transcriptionally spliced introns, PTS introns), which may play rapidly responding to changes environmental signals. However, mechanism underlying post-transcriptional regulation during initial light exposure young, etiolated seedlings remains elusive. In this study, we used FLEP-seq2, Nanopore-based sequencing technique, analyze nuclear RNAs Arabidopsis ( thaliana ) under different conditions found numerous light-responsive introns. We also single-nucleus (snRNA-seq) profile transcripts single investigate distribution across distinct cell types. established light-induced are predominant mesophyll cells seedling de-etiolation following light. further demonstrated involvement splicing-related factor A. PROTEIN ARGININE METHYLTRANSFERASE 5 (AtPRMT5), working concert with E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), critical repressor signaling pathways. these two proteins orchestrate events facilitate chloroplast photosynthesis, morphogenesis response ever-changing conditions. These findings provide crucial insights into intricate mechanisms acclimation at cell-type level.