A transient radial cortical microtubule array primes cell division in Arabidopsis

• DOI: 10.1073/pnas.2320470121
• Publication Date: 2024-07-11T17:50:29Z
• Authors: Isaty Melogno; Shogo Takatani; Paula Llanos; Coralie Goncalves; Chie Kodera; Marjolaine Martin; Claire Lionnet; Magalie Uyttewaal; Martine Pastuglia; Christophe Trehin; David Bouchez; Jacques Dumais; Olivier Hamant
• Keywords: Cell polarity

Although the formation of new walls during plant cell division tends to follow maximal tensile stress direction, analyses individual cells over time reveal a much more variable behavior. The origin such variability as well exact role interphasic microtubule behavior before have remained mysterious so far. To approach this question, we took advantage Arabidopsis stem, where pattern is both highly anisotropic and stable. cortical microtubules (CMTs) generally align with stress, detected specific window, ca. 3 h division, form radial CMTs. This array organization preceded preprophase band (PPB) formation, transient CMT predicting position future plane. It was observed under different growth conditions not related geometry or polar auxin transport. Interestingly, correlated well-documented increase cytoplasmic accumulation division. prolonged in trm678 mutant, CMTs are unable PPB. Whereas plane orientation noisier, found that symmetry contrast less between daughter cells. We propose “radial step” reflects trade-off robustness for two essential attributes: orientation. involves “reset” stage G2, an increased transiently disrupts alignment tissue stress.