Transcriptional profiles associated with aging and middle age-onset caloric restriction in mouse hearts

• DOI: 10.1073/pnas.232308999
• Publication Date: 2002-11-12T18:44:20Z
• Authors: Cheol-Koo Lee; David B. Allison; Jaap Brand; Richard Weindruch; Tomas A. Prolla
• Keywords: Senescence

To provide a global analysis of gene expression in the aging heart, we monitored 9,977 genes simultaneously 5- and 30-month-old male B6C3F 1 mice by using high-density oligonucleotide microarrays several statistical techniques. Aging was associated with transcriptional alterations consistent metabolic shift from fatty acid to carbohydrate metabolism, increased extracellular matrix genes, reduced protein synthesis. Caloric restriction (CR) started at 14 months age resulted 19% inhibition age-related changes expression. Interestingly, CR also preserved endogenous DNA damage, decreased innate immune activity, apoptosis modulation, marked cytoskeletal reorganization. These observations evidence that heart is specific alterations, initiated middle may retard inducing profound reprogramming.