DNA polymerase delta governs parental histone transfer to DNA replication lagging strand

• DOI: 10.1073/pnas.2400610121
• Publication Date: 2024-05-07T16:46:52Z
• Authors: Congcong Tian; Qin Zhang; Jing Jia; Jiaqi Zhou; Ziwei Zhang; Srinivasu Karri; Jiuhang Jiang; Quinn Dickinson; Yuan Yao; Xiaorong Tang; Yuxin Huang; Ting Guo; Ziwei He; Zheng Liu; Yuan Gao; Xinran Yang; Yuchun Wu; Kui Ming Chan; Daoqin Zhang; Junhong Han; Chuanhe Yu; Haiyun Gan
• Keywords: Replication; Lagging; DNA polymerase delta; DNA polymerase II; Replisome

Chromatin replication is intricately intertwined with the recycling of parental histones to newly duplicated DNA strands for faithful genetic and epigenetic inheritance. The transfer occurs through two distinct pathways: leading strand deposition, mediated by polymerase ε subunits Dpb3/Dpb4, lagging facilitated MCM helicase subunit Mcm2. However, mechanism facilitation Mcm2 transferring while moving along remains unclear. Here, we show that deletion Pol32, a nonessential major lagging-strand δ, results in predominant histone H3–H4 during replication. Biochemical analyses further demonstrate Pol32 can bind both vivo vitro. interaction disrupted mutation binding domain within Our findings identify δ as critical chaperone downstream Mcm2, mediating