Induction of Colonies of Hemoglobin-Synthesizing Cells by Erythropoietin In Vitro

• DOI: 10.1073/pnas.68.7.1542
• Publication Date: 2006-05-31T06:29:54Z
• Authors: John R. Stephenson; Arthur A. Axelrad; David L. McLeod; Mona M. Shreeve
• Keywords: Electrophoresis; Male; Erythrocytes; Iron; C3HF (C3HB,ZB); Bone Marrow Cells; Cell Line; Hemoglobins; Mice; 03 medical and health sciences; Fetus; Culture Techniques; Tissue Culture:; Leukocytes; Animals; Citrates; Strains: C3H/BI; Erythropoietin; 0303 health sciences; Staining and Labeling; Iron Isotopes; Organs:; Radiation:; Liver; Peroxidases; Embryology:; Morphology:; Autoradiography; Female

A culture method has been developed in which erythroid colonies are produced vitro from hemopoietic cells the livers of 13-day fetuses C3H(f)/Bi mice. Heme synthesis by cultures was correlated with presence these colonies, and hemoglobin shown to be electrophoretically normal. The individual were identified as since they erythropoietin-dependent, positively stained histochemical "Lepehne" procedure for hemoglobin, labeled (59)Fe radioautography. Evidence is presented that development under separate control granulocytic found same cultures.