Cellular transformation and differentiation. Effect of Rous sarcoma virus transformation on sulfated proteoglycan synthesis by chicken chondrocytes.

• DOI: 10.1073/pnas.74.10.4173
• Publication Date: 2006-05-31T07:23:05Z
• Authors: M Muto; M Yoshimura; M Okayama; A Kaji
• Keywords: Rous sarcoma virus; Chondroitin sulfate proteoglycan

Incorporation of sulfate into sulfated proteoglycans by isolated chicken chondrocytes was inhibited up to 74% transformation with the Rous sarcoma virus, and a similar inhibitory effect observed on acetate incorporation chondroitin sulfate. Slower sedimenting appear after viral transformation. The ratio 4-sulfate 6-sulfate in these slower different from that normal chondrocytes, but chain lengths glycosaminoglycans produced transformed were not significantly different. Chondrocytes also infected temperature-sensitive mutant RSV, ts LA24, which has lesion transforming gene. Hyaluronic acid production cells increased, proteoglycan only at permissive temperature.