Mutagenesis in S49 mouse lymphoma cells: induction of resistance to ouabain, 6-thioguanine, and dibutyryl cyclic AMP.

• DOI: 10.1073/pnas.74.2.679
• Publication Date: 2006-05-31T07:25:45Z
• Authors: U Friedrich; P Coffino
• Keywords: Ethyl methanesulfonate

The effects of mutagens on three genetic markers--resistance to ouabain, 6-thioguanine, and dibutyryl cyclic AMP (Bt2cAMP), were investigated in a mouse lymphoma cell line, S49. Nitrosoguanidine, ethyl methanesulfonate, ICR 191, x-rays used. Mutagen-specific responses seen. Ouabain resistance was induced by nitrosoguanidine, but not 191. 191 6-thioguanine more efficiently than did nitrosoguanidine; the converse true Bt2cAMP. relative frequency biochemically distinguishable subtypes mutants resistant Bt2cAMP characteristic mutagen used generate them. results can be interpreted as follows: nitrosoguanidine methanesulfonate frequently, rarely, give rise DNA base sequence changes that result structurally altered functional proteins. This type change is required for induction ouabain certain classes Resistance other from lead extensive alteration protein structure or expression; these are x-rays.