Gamma heavy chain disease in man: translation and partial purification of mRNA coding for the deleted protein.

• DOI: 10.1073/pnas.75.10.4774
• Publication Date: 2006-05-31T07:37:50Z
• Authors: A Alexander; D Barritault; J Buxbaum
• Keywords: Coding region; Genetic Code

Lymphoid cells obtained from the peripheral blood of a patient with heavy chain disease have been established in long-term culture. They continue to produce protein antigenically identical deleted gamma3 found patient's serum. The availability cell line has made it possible analyze mRNA coding for this protein. primary vitro translation product is 1500-2000 daltons larger than polypeptide portion cytoplasmic or secreted and methionine at amino terminus. sediments 15.5 S on sucrose gradients therefore appears be smaller 17S message normal-sized mouse gamma chains. It contains base sequence that codes hydrophobic amino-terminal peptide not There was no evidence synthesis translatable light by these cells. present data suggest results somatic genetic event gave rise bearing normal aminoterminus sensitive limited proteolytic digestion. serum thus begin hinge region but, fact, initiation site.