Organization of the recA gene of Escherichia coli.

• DOI: 10.1073/pnas.77.1.313
• Publication Date: 2006-05-31T08:04:56Z
• Authors: T Horii; T Ogawa; H Ogawa
• Keywords: Coding region

The restriction map of a BamHI DNA fragment that contains the recA gene Escherichia coli has been established and large portion fragment's nucleotide sequence determined. coding region 1059 residues encodes single protein 353 amino acid residues. first five NH2 terminus agrees with predicted from except for absence formylmethionine in purified protein. Immediately after sequence, there is G+C-rich dyad symmetry followed by an A+T-rich sequence. These could signal termination transcription. site initiation synthesis vitro messenger RNA determined analysis 5' [gamma-32P]ATP-labeled transcripts. promoter shos high degree sequences commonly found recognition binding sites polymerase.